Composite

Part:BBa_K912017

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-01)


BBa_J23114(weak) promoter/RBS+ Aspergillus Niger Phytase

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to constitutively produce aspergillus niger phytase. This phytase acts to break down phytic acid into inositol, releasing chelated iron and other micronutrients.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1410
    Illegal BamHI site found at 590
    Illegal XhoI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None